ProteaseGuru 0.1.1

.NET 8.0

dotnet add package ProteaseGuru --version 0.1.1

NuGet\Install-Package ProteaseGuru -Version 0.1.1

This command is intended to be used within the Package Manager Console in Visual Studio, as it uses the NuGet module's version of Install-Package.

<PackageReference Include="ProteaseGuru" Version="0.1.1" />

For projects that support PackageReference, copy this XML node into the project file to reference the package.

<PackageVersion Include="ProteaseGuru" Version="0.1.1" />
                    

                            Directory.Packages.props

<PackageReference Include="ProteaseGuru" />
                    

                            Project file

For projects that support Central Package Management (CPM), copy this XML node into the solution Directory.Packages.props file to version the package.

paket add ProteaseGuru --version 0.1.1

The NuGet Team does not provide support for this client. Please contact its maintainers for support.

#r "nuget: ProteaseGuru, 0.1.1"

#r directive can be used in F# Interactive and Polyglot Notebooks. Copy this into the interactive tool or source code of the script to reference the package.

#:package ProteaseGuru@0.1.1

#:package directive can be used in C# file-based apps starting in .NET 10 preview 4. Copy this into a .cs file before any lines of code to reference the package.

#addin nuget:?package=ProteaseGuru&version=0.1.1
                    

                            Install as a Cake Addin

#tool nuget:?package=ProteaseGuru&version=0.1.1
                    

                            Install as a Cake Tool

The NuGet Team does not provide support for this client. Please contact its maintainers for support.

ProteaseGuru: Free and Open-Source Tool for In Silico Database Digestion

Download the current version here.

ProteaseGuru is a in silico digestion tool for the planning of bottom-up proteomic experiments. ProteaseGuru allows for the digestion of one or more protein databases with as many proteases as desired. Results of the various proteolytic digests can be visualized with histograms as well as protein sequence coverage maps.

Check out the wiki page for software details!

Major Features

Ability to digest more than one database for application with multi-species samples such as xenografts, virally infected host cells and microbiome samples.
In silico digestion with as many proteases as desired!
Ability to define custom proteases for digestion.
Uniqueness of peptide sequences are determined both within a database and across all databases being analyzed.
Visualization of whole proteome digestion results is enabled by the generation and ability to export histograms.
Search for your proteins of interest and view their in silico digestion with a sequence coverage map with PTM and variant annotation!

System Requirements

Environment:
- 64-bit operating system
- .NET Core 8.0:
  - Windows: https://dotnet.microsoft.com/en-us/download/dotnet/thank-you/sdk-8.0.401-windows-x64-installer
  - macOS, x64 Intel processor: https://dotnet.microsoft.com/en-us/download/dotnet/thank-you/sdk-8.0.401-macos-x64-installer
  - macOS, ARM Apple Silicon processor: https://dotnet.microsoft.com/en-us/download/dotnet/thank-you/sdk-8.0.401-macos-arm64-installer
  - Linux: https://learn.microsoft.com/dotnet/core/install/linux?WT.mc_id=dotnet-35129-website
Note that ProteaseGuru only works on Windows at this time.
8 GB RAM recommended

Database Requirements

UniProt .XML or .fasta format; may be used in compressed (.gz) format.

mzLib

mzLib is a nuget package that we created as an all-purpose toolchest for mass-spec data analysis and many of its functions provide the tools for MetaMorpheus. mzLib is freely available for use in mass-spec applications. You do not need to download mzLib separately to run MetaMorpheus; it is already included.

References

Product	Compatible and additional computed target framework versions.
.NET	net8.0 is compatible. net8.0-android was computed. net8.0-browser was computed. net8.0-ios was computed. net8.0-maccatalyst was computed. net8.0-macos was computed. net8.0-tvos was computed. net8.0-windows was computed. net8.0-windows7.0 is compatible. net9.0 was computed. net9.0-android was computed. net9.0-browser was computed. net9.0-ios was computed. net9.0-maccatalyst was computed. net9.0-macos was computed. net9.0-tvos was computed. net9.0-windows was computed. net10.0 was computed. net10.0-android was computed. net10.0-browser was computed. net10.0-ios was computed. net10.0-maccatalyst was computed. net10.0-macos was computed. net10.0-tvos was computed. net10.0-windows was computed.

Product

.NET

Compatible target framework(s)

Included target framework(s) (in package)

Learn more about Target Frameworks and .NET Standard.

net8.0
- mzLib (>= 1.0.564)
- Nett (>= 0.15.0)
- TopDownProteomics (>= 0.0.287)
net8.0-windows7.0
- mzLib (>= 1.0.564)
- Nett (>= 0.15.0)
- OxyPlot.Core (>= 2.0.0)
- OxyPlot.Wpf (>= 2.0.0)
- TopDownProteomics (>= 0.0.287)

NuGet packages

This package is not used by any NuGet packages.

GitHub repositories

This package is not used by any popular GitHub repositories.

Version	Downloads	Last Updated
0.1.1	56	7/19/2025